Physiology of Neuronal Cell Signaling Pathways
PhD (1994-1998) Louisiana State University
Post-doctoral researcher (1998-1999) Louisiana State University
Post-doctoral researcher (1999-2003) Colorado State University
Assistant Professor, Special Track (2003-2004) Colorado State University
Assistant Professor (2004) University at Buffalo,
Associate Professor (2011-present) University at Buffalo
My lab studies the physiology of signal transduction pathways and the regulation of these pathways in neuronal systems. We focus on peripheral sensory systems, primarily the taste system. Chemical sensory systems, which are comprised of olfaction and taste, play important roles in feeding, territorial recognition and social interactions. The taste sensory system is used to determine whether potential food items will be ingested or rejected while the olfactory system is used in a multitude of behaviors such as kin recognition and mate selection. The taste system is extremely heterogeneous and is made up of multiple cell types that depend on multiple signaling pathways to detect stimuli. Some stimuli interact with receptors that initiate second messenger cascades, while others interact directly with ion channels to cause a cellular response. As characterization of these cellular mechanisms continues, we can begin understanding how the brain gathers information about its surroundings. The long term goal of the lab is to begin understanding how signaling mechanisms are regulated within taste cells and how this regulation impacts the generation of the stimulus signal to the brain. We use molecular and physiological techniques, including patch clamp analysis and calcium imaging to investigate how signaling mechanisms in taste cells function.
Characterization of calcium buffering mechanisms in taste cells of mice
Characterization of the PLCb3 signaling pathway in taste cells
* Defining the role of WT1 in taste development and maintenance
Maliphol, A.B., Garth, D.J. and Medler, K.F. (2013) Diet-induced obesity reduces the responsiveness of the peripheral taste system. PLoS ONE (in press)
Toska, E., Shandilya, J., Medler, K.F., Goodfellow, S.J., and Roberts, S.G.E. (2013) Prohibitin is required for transcriptional repression by the WT1-BASP1 complex. Oncogene (in press)
Rebello, M.R., Maliphol, A.B., and Medler, K.F. (2013). Ryanodine receptors selectively interact with L type calcium channels in mouse taste cells. PLoS ONE 8(6):e68174
Toska, E., Campbell, H.A., Shandilya, J., Goodfellow, S.J., Shore, P., Medler, K.F., and Roberts, S.G.E. (2012) Repression of transcription by WT1-BASP1 requires the myristoylation of BASP1 and the PIP2-dependent recruitment of Histone Deacetylase. Cell Reports 2(3):462-9.
Rebello, M. R., Aktas, A and Medler, K.F. (2011) Expression of calcium binding proteins in mouse taste receptor cells. J. Histo & Cyto. 59(5): 530-539.
Goodfellow, S.J., Rebello, M.R., Toska, E., Zeef,L., Rudd, S., Medler, K.F., and Roberts, S.G.E. (2011) WT1 and its transcriptional cofactor BASP1 redirect the differentiation pathway of an established blood cell line. Biochemical Journal 435(1):113-25.
Medler, K.F. (2011) Multiple roles for TRPs in the taste system: Not your typical TRPs. Invited chapter in “The Transient receptor potential channels” for the Advances in Experimental Medicine and Biology 704:831-46. Editor: Dr. Md. Shahidul Islam. Published by Springer, Inc.
Medler, K.F. (2010) Calcium signaling in taste cells: Regulation Required. Invited referred review for Chemical Senses. 35(9):753-65
Starostik, M.R, Rebello, M.R., Cotter, K.A., Kulik, A. and Medler, K.F. (2010) Expression of GABAergic receptors in mouse taste receptor cells. PLoS ONE 5(10): e13639.
Rebello, M. R. and Medler, K.F. (2010) Ryanodine receptors selectively contribute to taste evoked responses in mouse taste receptor cells. Eur. J. Neurosci. 32(11): 1825-1835.
Szebenyi, S.A., Laskowski, A.I., and Medler, K.F. (2010) Sodium/calcium exchangers selectively regulate calcium signaling in mouse taste receptor cells. J. Neurophys. 104: 529-538.
Laskowski, A.I. and Medler, K.F. (2009) Sodium/calcium exchangers contribute to the regulation of cytosolic calcium levels in mouse taste cells. J. Physiol. 587(16): 4077- 4089.
Medler, K., and Kinnamon, S.C. (2004) Transduction channels in Taste Cells. In Transduction Channels in Sensory Cells Eds. Frings and Bradley.
Medler, K, Margolskee, R.F., and Kinnamon, S.C. (2003) Electrophysiological characterization of voltage-gated currents in defined taste cell types of mice. J. Neurosci. 23(7):2608-2617.
Medler, K and Gleason, E. (2002) Mitochondrial Ca2+ buffering regulates synaptic transmission between retinal amacrine cells. J. Neurophysiol. 87: 1426-1439.
Kreimborg, K., Lester, M.,
Medler, K and Gleason, E. (2001) Group I
metabotropic glutamate receptors are expressed in the
chicken retina and by cultured retinal amacrine cells. J.
Neurochem. 77: 452-465.