BIO 402/502
Advanced Cell & Developmental Biology I
Fall 2012
Lectures: M, W, F 11:15am - 12:25pm ; NSC 218
Exams: September 27th, October 25th and November 17 from7pm - 9pm; NSC 215
Instructors: Professors Ron Berezney (Course Co-ordinator), Paul Cullen, and Steve Free
Textbooks: Molecular Biology of the Cell (Alberts et al., 5th edition, Garland Science, 2008) Reference Reading: Cell & Molecular Biology (Karp, 5th or 6th edition, John Wiley, 2008/10). Reading assignments will be from Alberts and/or Karp and/or additional readings provided by your instructor. Alberts is generally more comprehensive and cover cellular aspects of developmental biology for Section IV of the course. Karp is often superior in writing style and simplicity of explanations.
Course Evaluation: Grading will be based on four written in-class exams. Each of the 4 exams will count 25% of the final grade. The first 3 exams will be in NSC 215 from 7-9 PM on the designated dates. The last exam will be held during the scheduled time and room assigned for final exams. The exams will be composed of questions with short to medium length answers as well as possibly longer essay questions. Each instructor will devise his own exam and provide information to the students as to the exam format. Graduate and undergraduate students will take the same exam and be evaluated separately.
Advise:
Take good notes. Students need to fully understand the lectures, e.g., the power point slides.
Read and re-read the textbook assignments and other texts as necessary to fully understand the topics and to correlate with the lectures and power point slides.
ASAP read Chapters 2 & 3 of Karp or Alberts (also basic information on DNA/RNA in other chapters) to review basic biochemistry. If you detect significant deficiencies, see Dr. B.
BIO 402/502 Advanced Cell Biology
COURSE OUTLINE
Section I Cell Biology Methods; Biomembranes; Electron Transport & ATP Synthesis; Membrane Proteins; Cell Surface Receptors and Signal Transduction
Dr. Berezney (11 lectures, August 29th- September 23rd)
| Experimental Methods in Cell Biology | (Berezney; 4 lect, Aug. 29th, 31st, Sept 2th, 7th |
| Membrane Structure and Dynamics | (Berezney; 1 lect, Sept 9th) |
| Electron Transport and Oxidative Phosphorylation | (Berezney; 2 lect, Sept 12th & 14th) |
| Cell Surface Receptors and Signal Transduction | (Berezney; 1 lect, Sept 16th) |
| G-protein linked Cell-Surface Receptors | (Berezney; 1 lect, Sept 19th) |
| Protein-kinase linked Cell-Surface Receptors | (Berezney; 2 lect, Sept 21st, Sept 23rd) |
| EXAM 1 - September 27th | |
Dr. Cullen's Lecture Page
Section II - Protein Secretion, Trafficking & Degradation; Signal Transduction; Cytoskeleton & Cell Motility; Extracellular Matrix & Cell-Cell Interactions
Dr. Cullen (11 lectures, September 26th - October 19th)
Dr. Cullen's Lecture Material Page
| Protein Delivery to the ER, Golgi, Exocytosis | (Cullen; 1 lect, Sept. 26th) |
| Protein Glycosylation and unfolding | (Cullen; 1 lect, Sept. 28th) |
| Golgi, Snares, COPs and Arfs | (Cullen; 1 lect. Sept. 30th) |
| PI Signaling and Vesicle Identity | (Cullen; 1 lect, Oct. 3rd) |
| Protein Targeting: Peroxisome/Mitochondria | (Cullen; 1 lect, Oct. 5th) |
| Cell Polarity - Actin Cytoskeleton | (Cullen; 1 lect, Oct. 7th) |
| Cell Polarity - Microtubules | (Cullen; 1 lect, Oct. 10th) |
| Origins of Cell Adhesion | (Cullen; 1 lect, Oct. 12th) |
| Cell Adhesion Molecules, Integrins and Mucins and the Extracellular Matrix |
(Cullen; 1 lect, Oct. 14th) |
| Cell Polarity Regulation and Signal Transduction | (Cullen; 1 lect, Oct. 17th) |
| Genomics Approaches to Cell Biology | (Cullen; 1 lect, Oct. 19th) |
| EXAM 2 - October 25th | |
Section III - Developmental Systems in Cell Biology Dr. Free (10 lectures, October 21st – November 11th)
| Developmental strategies - maternal inheritance and regulative development | (Free; 1 lect, Oct. 21st) |
| Early Drosophila development - pattern formation - maternal and gap genes | (Free; 1 lect, Oct. 24th) |
| Drosophila development - pair rule and segmentation genes | (Free; 1 lect, Oct. 26th) |
| Drosophila nervous system and eye development - cell/cell communications | (Free; 1 lect, Oct. 28th) |
| Development of the C. elegans vulva - cell/cell communications | (Free; 1 lect, Oct. 31st) |
| Formation of the vertebrate limb -pattern formation in 3D | (Free; 1 lect, Nov. 2nd) |
| Development of vertebrate urogenital tract | (Free; 1 lect, Nov. 4th) |
| Metamorphosis in insects and vertebrates | (Free; 1 lect, Nov. 7th) |
| Importance of cell migrations - primitive streak to brain development | (Free; 1 lect, Nov. 9th) |
| Apoptosis | (Free; 1 lect, Nov. 11th) |
| EXAM 3 - November 17th | |
Section IV
- Cell Nucleus and Genomic Function; Cell Cycle & the Regulation of Cell Proliferation
Dr. Berezney (10 lectures, November 14th- December 9th)
| Topics | Date |
| The Cell Nucleus and its Genome | (Berezney; 1 lect, Nov. 14th) |
| Nuclear Envelope, NPC, Nucleolus and Nuclear Matrix | (Berezney; 1 lect, Nov. 16th) |
| Replication/Transcription/Splicing in the Nucleus | (Berezney; 2 lect, Nov. 18th, 21st) |
| Nuclear Import/Export and Targeting | (Berezney; 2 lect, Nov. 28th, 30th) |
| Cell Cycle and the Regulation and Cell Proliferation | (Berezney; 4 lect, Dec. 2nd, 5th, 7th, 9th) |
| EXAM 4 - Monday, December 12 in NSC 218 from 11:45 - 1:45 pm. | |
End