BIO 402/502
Advanced Cell & Developmental Biology I
Fall 2009
Lectures: M, W, F 11:15am - 12:25pm ; NSC
222
Exams: 7pm - 9pm; Hochstetter 114
Instructors: Professors Ron Berezney (Course Co-ordinator, Paul Cullen, and Steve Free
Textbooks: Molecular Biology of the Cell (Alberts et al., 5th edition, Garland Science, 2008) Reference Reading: Cell & Molecular Biology (Karp, 4th or 5th edition, John Wiley, 2005/8). Reading assignments will be from Alberts and/or Karp and/or additional readings provided by your instructor
Course Evaluation: Grading will be based on four written in-class exams. The first 3 exams will be in Hochstetter 114 from 7-9 PM on the designated dates. The last exam will be held during the scheduled time and room assigned for final exams. The exams will be composed of questions with short to medium length answers as well as essay questions. Graduate and undergraduate students will take the same exam and be evaluated separately.
BIO 402/502 Advanced Cell Biology
COURSE OUTLINE
Section I - Cell Biology Methods; Biomembranes; Electron Transport & ATP Synthesis; Membrane Proteins; Cell Surface Receptors and Signal Transduction Drs. Berezney (10 lectures, August 31 - September 23)
| Experimental Methods in Cell Biology | (Berezney; 3 lect, Aug. 31st, Sept. 2, Sept. 4) |
| Membrane Structure and Dynamics | (Berezney; 1 lect, Sept 9 ) |
| Electron Transport and Oxidative Phosphorylation | (Berezney; 2 lect, Sept 11, Sept. 14) |
| Properties of Membrane Proteins & Lipid Rafts | (Berezney; 1 lect, Sept 16) |
| Cell Surface Receptors and Signal Transduction | (Berezney; 1 lect, Sept 18) |
| G-protein linked Cell-Surface Receptors | (Berezney; 1 lect, Sept 21) |
| Protein-kinase linked Cell-Surface Receptors | (Berezney; 1 lect, Sept 23 ) |
| Exam 1 (Proctor: Cullen, September 29) |
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Section II - Protein Secretion, Trafficking & Degradation; Signal Transduction; Cytoskeleton & Cell Motility; Extracellular Matrix & Cell-Cell Interactions Dr. Cullen (12 lectures, September 25 - October 23)
| Protein Delivery to the ER, Golgi, Exocytosis | (Cullen; 1 lect, Sept. 25) |
| Protein Glycosylation and unfolding | (Cullen; 1 lect, Sept. 30) |
| Golgi, Snares, COPs and Arfs | (Cullen; 1 lect. Oct. 2) |
| PI Signaling and Vesicle Identity | (Cullen; 1 lect, Oct. 5) |
| Protein Targeting: Peroxisome/Mitochondria | (Cullen; 1 lect, Oct. 7) |
| Cell Polarity - Actin Cytoskeleton | (Cullen; 1 lect, Oct. 9) |
| Cell Polarity - Microtubules | (Cullen; 1 lect, Oct. 12) |
| Origins of Cell Adhesion | (Cullen; 1 lect, Oct. 14) |
| Cell Adhesion Molecules, Integrins and Mucins | (Cullen; 1 lect, Oct. 16) |
| The Extracellular Matrix | (Cullen; 1 lect, Oct. 19) |
| Cell Polarity Regulation and Signal Transduction | (Cullen; 1 lect, Oct. 21) |
| Genomics Approaches to Cell Biology | (Cullen; 1 lect, Oct. 23) |
| Exam 2 (Proctor: Berezney - October 29) |
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Section III - Developmental Systems in Cell Biology Dr. Free (10 lectures, October 26th – November 16th)
| Developmental strategies - maternal inheritance and regulative development | (Free; 1 lect, Oct. 26) |
| Early Drosophila development - pattern formation - maternal and gap genes | (Free; 1 lect, Oct. 28) |
| Drosophila development - pair rule and segmentation genes | (Free; 1 lect, Oct. 30) |
| Drosophila nervous system and eye development - cell/cell communications | (Free; 1 lect, Nov. 2) |
| Development of the C. elegans vulva - cell/cell communications | (Free; 1 lect, Nov. 4) |
| Formation of the vertebrate limb -pattern formation in 3D | (Free; 1 lect, Nov. 6) |
| Development of vertebrate urogenital tract | (Free; 1 lect, Nov. 9) |
| Metamorphosis in insects and vertebrates | (Free; 1 lect, Nov. 11) |
| Importance of cell migrations - primitive streak to brain development | (Free; 1 lect, Nov. 13) |
| Apoptosis | (Free; 1 lect, Nov. 16) |
| Exam 3 (Proctor: Free, November 19) |
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Section IV - Cell Nucleus and Genomic Function; Cell Cycle & the Regulation of Cell Proliferation, Dr. Berezney (9 lectures, November 18th- December 11th)
| Topics | Date |
| The Cell Nucleus and its Genome | (Berezney; 1 lect, Nov. 18) |
| Nuclear Envelope, NPC, Nucleolus and Nuclear Matrix | (Berezney; 1 lect, Nov. 20) |
| Replication/Transcription/Splicing in the Nucleus | (Berezney; 3 lect, Nov. 23, 30) |
| Nuclear Import/Export and Targeting | (Berezney; 3 lect, Dec. 2, 4) |
| Cell Cycle and the Regulation and Cell Proliferation | (Berezney; 3 lect, Dec. 7, 9, 11) |
Exam 4 |
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End